Structure specific nucleases that act in DNA replication, repair and recombination actively mold their DNA while transforming their own structure to achieve precise cleavage of their cognate DNA and avoid the deleterious cleavage of noncognate DNA.
Transcriptome-scale RNA imaging and lifetime measurements reveal that the E. coli transcriptome is spatially organized and that this organization modulates the post-transcriptional fate of bacterial mRNAs.
A novel computation tool for microbial community modeling predicts the evolution and diversification of E. coli in laboratory evolution experiments and gives insight into the underlying metabolic processes.
Experiments on E. coli show that multiple mechanisms contribute to extreme resistance to ionizing radiation in bacteria, with mutations to three genes for DNA repair having a prominent role in one evolved population.
A transport mechanism is uncovered in the major drug-efflux system in E. coli involving two remote alternating-access conformational cycles, which could provide the basis for the development of allosteric inhibitors against multidrug resistance.