Secretion and fibrillogenesis of collagen-I are separately controlled molecular pathways in the fibroblast, where surprisingly endocytic recycling is required for fibril formation and is increased in fibrotic fibroblasts.

Single-molecule tracking at scale, analyzing millions of cells and thousands of compounds per day, demonstrates that measuring protein motion provides mechanistic insights relevant to drug discovery.

Membrane atg8ylation but not canonical autophagy control retromer-dependent endosomal sorting of cargo, including glucose transporter GLUT1, of general import and of significance for control of tuberculosis.