Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.

A new pipeline of electron microscopy techniques reduces the time required to visualize genetically targeted neurons and their connections by two orders of magnitude.

The structure of a large enzyme complex involved in methanogenesis was determined at high resolution by electron cryo-microscopy.

In situ X-ray imaging of biological tissue samples in staining solution while they are being processed for electron microscopy reveals novel insights into the diffusion-reaction kinetics, tissue mechanics, and staining quality.

A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.

'Cellular Electron Microscopy 500,000 images' (CEM500K) is a highly heterogeneous, information-rich, non-redundant, unlabeled EM dataset curated to pre-train DL algorithms for better model generalization on EM segmentation tasks.

Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.

The first structure of the human epithelial sodium channel provides vital insight into the assembly, stoichiometry and molecular mechanism of the ion channel central to sodium homeostasis.

The structure of a large hydrogenase complex involved in the production of methane by microbes has been determined with cryo-electron microscopy.

New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.