Sox2 transcription is not correlated with spatial proximity of its essential regulatory enhancer in embryonic stem cells, suggesting gene transcription is not limited to periods of direct enhancer-promoter contact.
Multiple enhancers in physical proximity can reinforce shared transcriptional 'hubs' to preserve their transcriptional output, providing a buffer during environmental stresses and genetic perturbations to preserve phenotypic robustness.
FGF signalling is responsible for priming the developmental enhancer ZRS across the distal limb mesenchyme during development, allowing ETS factors to modulate its activity through balancing histone acetylation.
Transcription-factor-dependent noncoding RNA transcription illuminates components of a transcription-factor-dependent gene regulatory network that includes enhancer-associated long noncoding RNAs and is necessary for cardiac rhythm.
The sharp expression pattern driven by a classic, simple animal enhancer is determined by multiple molecular mechanisms, not only cooperative binding of the activating transcription factor as was previously thought.