Computational simulations of actin network growth at the cell leading edge, combined with careful high-speed video microscopy measurements of leading edge shape fluctuations, suggest that Arp2/3 branching of the actin network occurs at an optimal angle to minimize fluctuations.
David O Ajasin, Vasudev R Rao ... Vinayaka R Prasad
Effect of CCL2 on HIV-1 replication is mediated by mobilization of ALIX from F-actin leading to enhanced virion release and improved fitness and this requires the Gag late motif, LYPX.
Johannes Felix Stortz, Mario Del Rosario ... Sujaan Das
Formin-2 controls spatiotemporal polymerisation of actin filaments, a common mechanism used by apicomplexans for effective segregation of essential chloroplast-like organelles called apicoplasts, and additionally for daughter formation in Plasmodium falciparum.
Katarzyna Szymanska, Karsten Boldt ... Colin A Johnson
Interacting MKS1 and UBE2E1 regulate canonical Wnt signaling through modulation of beta-catenin levels at the base of primary cilium, providing new insights into ciliopathy disease mechanisms.
Functional molecular analysis of cytokinesis in Caenorhabditis elegans four-cell embryos reveals cell-type-specific variation in the fundamental dependence on a robust f-actin cytoskeleton for successful cell division, mediated by both cell-intrinsic and -extrinsic pathways.
During infection, L. pneumophila reverses the ATP-synthase activity of the mitochondrial FOF1-ATPase to ATP-hydrolase activity in a type 4 secretion-dependent manner to prevent early cell death, thereby preserving its replication niche.
Single-molecule TIRF microscopy, biochemical assays and mathematical modeling demonstrate how actin-branch nucleation by the Arp2/3 complex is coordinately regulated by two biochemically distinct activators.
