12 results found
    1. Neuroscience

    Excitatory and inhibitory synapse reorganization immediately after critical sensory experience in a vocal learner

    Ziqiang Huang, Houda G Khaled ... Richard HR Hahnloser
    Sensory acquisition of a motor target is associated with rapid removal of excitatory synapses and with rapid insertion of inhibitory synapses in the main song control area of songbirds.
    1. Neuroscience

    The comprehensive connectome of a neural substrate for ‘ON’ motion detection in Drosophila

    Shin-ya Takemura, Aljoscha Nern ... Ian A Meinertzhagen
    Connectomic analysis identifies the complex circuits of a visual motion-sensing neuron that qualify them to generate direction-selective motion sensing signals using both Hassenstein-Reichardt and Barlow-Levick models.
    1. Neuroscience

    Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation

    Hiromi Tamada, Jerome Blanc ... Graham W Knott
    Cryo preservation of brain tissue reveals dendritic spines with thinner necks compared to those prepared with chemical fixation.
    1. Neuroscience

    Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation

    Natalya Korogod, Carl CH Petersen, Graham W Knott
    Snap-freezing of brain tissue reveals its true structure-showing that cells are less squashed together, and the connections between neurons are less enclosed than previously thought.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    HID-1 is required for homotypic fusion of immature secretory granules during maturation

    Wen Du, Maoge Zhou ... Tao Xu
    HID-1 is a novel player mediating homotypic fusion of immature secretory granules.
    1. Structural Biology and Molecular Biophysics

    A modular platform for automated cryo-FIB workflows

    Sven Klumpe, Herman KH Fung ... Julia Mahamid
    SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.
    1. Neuroscience

    A connectome of a learning and memory center in the adult Drosophila brain

    Shin-ya Takemura, Yoshinori Aso ... Louis K Scheffer
    Connectomic analysis in the fly's (Drosophila) learning and memory center identifies unique circuit motifs that have not been anticipated by over 30 years of extensive anatomical, experimental and theoretical studies.
    1. Structural Biology and Molecular Biophysics

    A cryogenic, coincident fluorescence, electron, and ion beam microscope

    Daan B Boltje, Jacob P Hoogenboom ... Sander den Hoedt
    Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.
    1. Neuroscience

    Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

    Rohan Gala, Daniel Lebrecht ... Armen Stepanyants
    BoutonAnalyzer software makes it possible to detect axonal boutons and measure their structural plasticity in long-term in vivo imaging experiments.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

    Helen R McPherson, Cedric Duval ... Robert AS Ariëns
    Fibrinogen, a key protein involved in blood coagulation, contains a disordered and previously poorly characterised region called αC that plays a critical role in fibrin fibre growth and clot stability.

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