Malaria parasites have simplified and adapted their mitochondrial metabolism to lack fatty acid synthesis but retain an unusual acyl carrier protein required for stability of the core iron-sulfur cluster biogenesis complex.
Cell biological, biochemical and structural studies reveal the mechanistic roles of two new mitochondrial iron-sulfur protein assembly factors, explaining the patho-biochemical defects of affected patients.
Structural analysis of the mitoribosomal small subunit with streptomycin reveals the molecular basis for antibiotic binding and new physiological components such as FeS clusters that assemble between mitochondria specific proteins.
Mitochondrial proteins Nfu1 and BolA3 mediate the transfer of preformed [4Fe-4S] clusters within the mitochondria to recipient proteins to shield clusters from oxidative damage during transfer.
Viktoria Désirée Paul, Ulrich Mühlenhoff ... Roland Lill
Two newly identified assembly factors for the ribosome-associated iron-sulfur protein Rli1 reveal a general mechanism for how the cytosolic iron-sulfur protein assembly (CIA) machinery recruits apoproteins.
Francesca D'Angelo, Elena Fernández-Fueyo ... Gregory Bokinsky
The intracellular network that distributes iron-sulfur clusters in bacteria is surprisingly 'plug and play' with iron-sulfur enzymes acquired from distantly related species, whereas the intracellular electron transfer network often needs plug adapters to connect with foreign enzymes.
X-ray crystal structures capture ATP-dependent chalcogenide exchange from selenocyanate at the nitrogenase Fe protein cluster in the absence of the MoFe protein, an unexpected result as the Fe protein cluster is not traditionally perceived as a site of substrate binding.
Ma Teresa Pellicer Martinez, Jason C Crack ... Nick E Le Brun
Molecular details of how the iron–sulfur cluster cofactor of a bacterial global iron regulatory protein simultaneously senses iron and O2 are revealed by mass spectrometry and spectroscopy.
Hsiang-Chun Chang, Jason Solomon Shapiro ... Hossein Ardehali
Mammalian cellular iron homeostasis and the maturation of the cytosolic iron sulfur cluster proteins are dependent on the mitochondrial protein import machinery.
