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    1. Plant Biology

    Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts

    Yusuke Kato, Hiroshi Kuroda ... Wataru Sakamoto
    Oxidative modification of Tryptophan residues in the reaction center protein D1 may be a key to drive the Photosystem II repair, likely enhancing accessibility of FtsH protease to D1.
    1. Structural Biology and Molecular Biophysics

    Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution

    Adrián Merino-Salomón, Jonathan Schneider ... Germán Rivas
    Not revised
    Reviewed Preprint v1
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    1. Genetics and Genomics

    A regulatory pathway that selectively up-regulates elongasome function in the absence of class A PBPs

    Yesha Patel, Heng Zhao, John D Helmann
    Balanced peptidoglycan synthesis requires regulators, including sigma-I and WalKR, that coordinate the diffusive action of class A PBPs and the directional motion of the MreB-directed elongasome.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq

    Liselot Dewachter, Julien Dénéréaz ... Jan-Willem Veening
    High-throughput single-cell based, genome-wide gene silencing screening (sCRilecs-seq) revealed that drugging the mevalonate pathway resensitizes amoxicillin-resistant pneumococci.
    1. Plant Biology

    A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis

    Rosa Pipitone, Simona Eicke ... Emilie Demarsy
    Serial-Block-Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘structure establishment’ and ‘chloroplast proliferation’ phases, each with differential protein and lipid regulation.
    1. Microbiology and Infectious Disease

    Transposon mutagenesis in Mycobacterium abscessus identifies an essential penicillin-binding protein involved in septal peptidoglycan synthesis and antibiotic sensitivity

    Chidiebere Akusobi, Bouchra S Benghomari ... Eric J Rubin
    PBP-lipo is an essential cell-wall synthesis enzyme in Mycobacterium abscessus that localizes to the septum and whose expression is required for normal cell growth and division, as well as mediating sensitivity to several antibiotics including the β-lactams, ampicillin, and amoxicillin.
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    A bacterial membrane sculpting protein with BAR domain-like activity

    Daniel A Phillips, Lori A Zacharoff ... Sarah M Glaven
    Uniform diameter and curvature of the outer membrane extensions and vesicles of Shewanella oneidensis are maintained by a bacterial Bin/Amphiphysin/Rvs (BAR) domain-like protein BdpA.
    1. Microbiology and Infectious Disease

    Molecular coordination of Staphylococcus aureus cell division

    Victoria A Lund, Katarzyna Wacnik ... Simon J Foster
    Morphological constraints dictate division mode in the human pathogen Staphylococcus aureus.
    1. Developmental Biology

    MMP14 cleaves PTH1R in the chondrocyte-derived osteoblast lineage, curbing signaling intensity for proper bone anabolism

    Tsz Long Chu, Peikai Chen ... Kathryn Song Eng Cheah
    MMP14 activity-mediated cleavage of the ectodomain of PTH1R modulates PTH signaling in the chondrocyte-derived osteoblast lineage, regulating osteogenesis and thereby bone metabolism, with therapeutic significance for bone-wasting diseases.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Two-step membrane binding by the bacterial SRP receptor enable efficient and accurate Co-translational protein targeting

    Yu-Hsien Hwang Fu, William Y C Huang ... Shu-ou Shan
    Single molecule microscopy combined with biochemical analyses show that a two-step lipid-binding mechanism of the SRP receptor balances the trade-off between speed and specificity during co-translational protein targeting.