Genetic lesions that compromise the ribosome P-stalk implicate direct signalling from the ribosome to the translation initiation factor eIF2 kinase GCN2 in the cellular response to amino acid starvation.
Activation of the integrated stress response by stalled translation elongation complexes attenuates neurodegeneration, and demonstrates a protective link between a decrease in the rate of translation initiation and defects in translation elongation.
Unloading the polymerase sliding clamp PCNA from DNA by Elg1 promotes recombination at the RTS1 replication fork barrier by limiting Fbh1 and Srs2 activity.
Sea3, a presumed subunit of the GATOR2 complex in fission yeast, is required for the GATOR1 function that attenuates TORC1 in parallel with the TSC complex and Gcn2 kinase.
Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
GCNA proteins comprise a previously unnoticed family of proteins that has been enriched in cells carrying a heritable genome since the invention of sexual reproduction in eukaryotes, and has had reproductive function for at least 600 million years.
Activation of the stress response pathway in young cells extends replicative lifespan, not by reducing global protein synthesis per se, but by Gcn4-mediated autophagy induction.
The percentage of a tumor’s genome with alterations in copy number is correlated with increased mortality across a range of tumor types and can be measured using a clinically approved sequencing assay.
CNApp is a novel and unique web-based software that enables the performance of comprehensive and integrative analysis of genomic copy number alterations to uncover new associations with patient-oriented relevance.
Biochemical analysis in Xenopus egg extracts reveals that the MutSα mismatch sensor retains the DNA-bound replication clamp to maintain a post-replicative temporal window permissive to strand-specific repair of mismatches.
