Focusing on calcium oscillations in relation to neuronal morphology both in vitro and in vivo, rationally designed GCaMP-X provides a simple solution to neuronal toxicity of conventional GCaMP in imaging applications involving prolonged and/or strong expression of calcium probes.

Common adeno-associated viral transduction procedures induce artefactual spatially confined Ca²⁺ waves in the hippocampus.

Protein engineering and large-scale screening has led to the development of sensitive red fluorescent indicators that detect neural activity with high sensitivity in various animal models.

Nanodomain clustering of cAMP effectors represents a novel mechanism of cAMP compartmentation within an oscillatory signaling circuit.

Salsa6f is a novel, ratiometric genetically encoded Ca²⁺ indicator that combines the power of ratiometric chemical Ca²⁺ indicators with the ability, when expressed transgenically, to image cellular Ca²⁺ signals amid complex tissue environments in vivo.

Simultaneous calcium imaging and cell-attached recording in GCaMP6 transgenic mice in vivo reveals spiking-fluorescence relationship that supports more refined inference of neuronal activities from calcium imaging data.

Microglial processes engage calcium signaling in response to hyper- or hypo-active shifts in neuronal activity.

Discrepancies between functional responses measured with electrophysiology and calcium imaging can be largely reconciled by considering the spike-to-calcium transfer function, neuronal sampling bias, and cluster merging artifacts during spike sorting.

Live cell imaging shows that the cAMP-sensor Epac2, a target of major antidiabetic drugs, is central to fusion pore control during insulin granule exocytosis.

Mesoscale cortical calcium activity correlating with single cortical and thalamic cell spiking reveal rich dynamics and support a novel approach for investigating in vivo functional networks in the mammalian brain.