A new genetically engineered mouse strain is described in which miRNA activity can be acutely and reversibly inhibited, showing that in a subset of adult tissues miRNA activity is dispensable under homeostatic conditions but becomes essential during tissue regeneration.
Genetic and biochemical analyses reveal that two stem-cell-specific microRNAs control stem cell fate decisions between pluripotency and differentiation through repressing Ago2, a key component of the microRNA machinery.
A comprehensive analysis of Tensin3 function in oligodendrogenesis links the fields of transcriptional control in oligodendrocyte lineage cells with morphogenetic changes and integrin signaling mediated cell survival.
Sandy Mattijssen, James R Iben ... Richard J Maraia
LARP4 protects mRNAs against deadenylation over a wide range of poly(A) lengths but with most apparent functional impact on short lengths known to sensitize PABP binding and mRNA stability.
A viral heliorhodopsin from Emiliania huxleyi virus 202 (V2HeR3) is a light-activated proton transporter, which has the potential to depolarize the host cells by light, possibly to overcome the host defense mechanisms or to prevent superinfection.
The utility of split GFP for tissue-specific visualization of ribosomes in live Caenorhabditis elegans demonstrates the link between ribosomes and microtubules.
The crystal structure of a mammalian protein kinase G reveals contacts between the regulatory and catalytic domains, indicates how cGMP binding alters domain conformations and thus domain:domain interactions, and informs a model for enzyme auto-inhibition and cooperative activation.
Matthew J Shurtleff, Morayma M Temoche-Diaz ... Randy Schekman
A cell-free reaction that reconstitutes the selective sorting of a miRNA into exosomes reveals an RNA-binding protein, YBX1, as a critical sorting factor.
