A single-molecule approach is able to discriminate the redundant binding conformations of cellulosomal dockerin based on differences in their mechanical properties.

The cancer-associated human ubiquitin ligase HUWE1 can adopt an auto-inhibited dimeric state, whose occupancy is regulated by competing intra- and intermolecular interactions of the dimerization region and by the tumor suppressor p14ARF.

Insight into the mechanism of DNA synthesis is provided by the crystal structures of DNA polymerase intermediates.

Cryo-EM studies reveal that incubation with ATP produces conformational intermediates of E. coli ATP synthase, in which the ε subunit is no longer in its autoinhibitory conformation.

An alternative iron storage system based on a 9.6 megadalton microbial protein compartment is able to sequester and store large amounts of iron.

RNA polymerase recruits the PcrA DNA helicase via a conserved interaction motif in order to remove R-loops and prevent conflicts with replication.

The McsB kinase is critical for protein quality control in Gram-positive bacteria, assembling a gated phosphorylation chamber during heat-shock conditions to selectively mark misfolded proteins for degradation.

Single-molecule F₁F_O studies show mutation-dependent pKa changes of both F_O half-channels, and proton translocation-dependent 11° ATP synthase-direction sub-steps, which support a Grotthuss proton transfer-dependent two-step F_O torque generating mechanism.

The conformational state of a structural protein in bacteria can vary, depending on the concentration level of potassium ions or the nucleotide bound to it.

First 3D-structure and first observation of organized protofilaments of MreB from a Gram-positive bacterium, suggesting a model where ATP drives the formation of dynamic pairs of MreB protofilaments on a lipid surface.