The alpha-synuclein fibril structure reported here buries residues 50-57 at the interface between its two protofilaments, suggesting that familial Parkinson's disease associated mutations in these residues lead to a structure not compatible with the one presented here.
Integrin and actin associated proteins are resolved into four layers within myofibril attachments, an architecture requiring balanced positive and negative regulation of integrin adhesion with integrated mechanotransduction and actin pathways.
Translationally active polysomes from mammalian cells/tissues are successfully separated with rapidity, high efficiency and exceptional reproducibility by a SEC-based approach, an accessible alternative to the conventional sucrose density gradient analysis.
Yeast cell size homeostasis is not controlled by a G1-specific mechanism alone but is likely to be an emergent property resulting from the integration of several mechanisms that coordinate cell and bud growth with division.
JNK pathway activity induces spermatogonial dedifferentiation under challenging conditions to maintain the germline stem cell pool and to endow it with potentially fitter cells that have increased proliferation.
Plexin controls the spatial distribution of synapses by locally inhibiting Rap2 small GTPase activity along the axon, and a Rap2 effector, TNIK, which also plays a key role in inhibiting synapse number.
Membrane potential recordings in awake mice from two genetically defined classes of neocortical inhibitory neurons reveal their distinct patterns of activity and unitary excitatory synaptic inputs, which are differentially modulated by behavior and local network activity.