Structural studies reveal the mechanism by which a HECT E3 ubiquitin ligase carries out E2-to-E3-to-substrate ubiquitin transfer and prioritizes target lysines for ubiquitination.

Diverse sophisticated phylogenetic analyses update the phylogeny of the Alphaproteobacteria and show that the parasitic Holosporales is a derived group within the Rhodospirillales order which comprises primarily free-living alphaproteobacteria.

Forward programming of human pluripotent stem cells with a combination of four transcription factors allows the production of functional hepatocytes.

The chromatin remodeler and tumor suppressor SMARCB1 acts to restrict superenhancer function to direct neural differentiation of embryonic stem cells while repressing bivalent gene activity in the pluripotent state.

HEC transcription factors control the timing of cell fate transitions in a dynamic stem cell system, allowing plants to adapt their developmental program to diverse environments.

Interaction of human embryonic stem cells (hESC) with human amniotic epithelial cells (hAEC) confers hESC a pluripotent potential that resembles the anteriorized epiblast, which is predisposed to form the neural ectoderm.

Using a lineage tracing model, it is demonstrated that adult murine hematopoiesis is contingent on the continuous output from HSCs, with quantitative contributions that are dependent on lineage-type and age.

Human induced pluripotent stem cells and embryonic stem cells express a similar set of proteins, but the levels of cytoplasmic and mitochondrial proteins vary, with concomitant effects on phenotypes including transport and mitochondrial function.

Complete neuronal circuits can be reconstructed, and whole eukaryotic cells imaged, at less than 10 nm isotropic resolution.

Tom1 is the ubiquitin ligase in yeast that anchors a protein quality control pathway that rapidly eliminates ribosomal proteins that fail to assemble into ribosomes.