583 results found
    1. Structural Biology and Molecular Biophysics

    Two forms of Opa1 cooperate to complete fusion of the mitochondrial inner-membrane

    Yifan Ge et al.
    An in vitro reconstitution assay reveals stoichiometric levels of the short form of Opa1 work together with the long form of Opa1 to mediate efficient and fast membrane pore opening.
    1. Structural Biology and Molecular Biophysics

    Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies

    Lars-Anders Carlson et al.
    In vitro reconstitution shows how HIV-1 Gag assemblies on membranes can package the RNA genome in the presence of a vast excess of competing cellular RNAs, and that selectivity and immature lattice assembly are deeply intertwined with one another.
    1. Cell Biology

    In vitro reconstitution of branching microtubule nucleation

    Ammarah Tariq et al.
    Purification of two conserved protein complexes, the γ-TuRC and Augmin, using a simple affinity technique, demonstrates that they are necessary and sufficient for the essential phenomenon of branching microtubule nucleation.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution

    Scott M Coyle, Wendell A Lim
    A systems level reconstitution of H-Ras GTPase signaling reveals a rich space of tunable dynamic outputs and clarifies the consequences of oncogenic perturbations to signaling.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters

    Inda Setyawati et al.
    Integral membrane subunits of energy-coupling factor transporters associate with and dissociate from each other as part of the transport mechanism.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin

    Charlotte F Kelley et al.
    In vitro reconstitution of the core focal adhesion proteins talin and vinculin with actin reveals lipid-dependent release of autoinhibition.
    1. Cell Biology

    Metformin extends C. elegans lifespan through lysosomal pathway

    Jie Chen et al.
    Metformin extends C. elegans lifespan through lysosome-dependent coordination of TORC1 and AMPK pathways.
    1. Cell Biology

    In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

    Weirui Ma et al.
    Binding of a multivalent RNA-binding protein to mRNAs that are able to form pervasive RNA–RNA interactions induces formation of mesh-like condensates, whereas binding of mostly structured mRNAs induces sphere-like condensates.
    1. Cell Biology

    Lissencephaly-1 is a context-dependent regulator of the human dynein complex

    Janina Baumbach et al.
    An in vitro reconstitution approach reveals context-dependent roles of Lissencephaly-1 in the regulation of dynein-dynactin behaviour on dynamic microtubules.
    1. Biochemistry and Chemical Biology
    2. Plant Biology

    Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis

    Satoshi Yamashita et al.
    The first de novo synthesis of polyisoprenoids correspond to natural rubber in vitro with the recombinant proteins is achieved by identification and cell-free translation-coupled reconstitution of the rubber biosynthetic machinery on rubber particles.

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