A biochemical reconstitution shows that the accumulation of Caulobacter crescentus ParB on DNA requires cytidine triphosphate and a closed DNA substrate in vitro.
Mark A McClintock, Carly I Dix ... Simon L Bullock
Reconstitution of dynein-based mRNA transport with purified components reveals a pivotal role of an RNA localisation signal in controlling the assembly and activity of the translocation machinery.
A cell-free biochemical assay for protein lipidation identifies the ER–Golgi intermediate compartment as a key early station in the formation of an autophagosome.
The architecture of the bacterial cytokinetic ring in cells and in artificial liposome reconstitutions has been described using electron microscopy, leading to a mechanism of constriction.
Biochemical and cell biological analyses reveal that the Astrin-SKAP complex acts to stabilize kinetochore-microtubule interactions through its intrinsic microtubule binding activity and its association with the Ndc80 complex, the core component of the kinetochore-microtubule interface.
A minimal cell-like system with defined geometry has been used to investigate the establishment and spatial control of a protein gradient that positions the bacterial cell division machinery.
Steven Henikoff, Srinivas Ramachandran ... Jorja G Henikoff
A stable tetrameric nucleosome occupies the central segment of each ∼120-bp budding yeast centromere in two rotational phases of both reflectional orientations in vivo.
