In vivo imaging reveals that gradually increased amount of glucose mediates the heterogeneous functional development of individual β-cells by activating its major downstream calcineurin/NFAT signaling pathway.
A high-throughput functional validation system for large numbers of candidate disease genes enables in vivo functional testing of specific gene variants.
Super-resolution STED microscopy is demonstrated for the first time in the deeply embedded mouse hippocampus in vivo, revealing direct evidence for an unprecedentedly high level of synapse remodeling in a brain structure closely associated with memory processing.
In vivo imaging of extracellular signal-regulated kinase activity reveals radial ERK activation patterns that are associated with cell cycle progression in the mouse epidermis.
Short, theta-bursts of action potential firing decrease the global excitability of CA1 pyramidal neurons, providing an internal mechanism which could regulate their allocation to memory engrams.
Changing the order in which presynaptic and postsynaptic cells are repeatedly activated can change what a mammalian visual cortex neuron communicates to downstream neurons.
A robot capable of automatically obtaining blind whole cell patch clamp recordings from multiple neurons simultaneously guides four interacting electrodes in a coordinated fashion, avoiding mechanical coupling in the brain.
We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.
Building on previous work (Grotjohann et al., 2012), low-light super-resolution microscopy has been performed on living transgenic Drosophila larvae and tissues.
