42 results found
    1. Plant Biology

    Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

    Ivan Kulich, Julia Schmid ... Jiří Friml
    Negative Gravitropic Response of roots (NGRs), pivotal for root gravitropic bending, are indispensable for the gravity-induced translocation of D6 protein kinase, a key regulator of PIN3 auxin efflux carrier activity.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Light-inducible protein degradation in E. coli with the LOVdeg tag

    Nathan Tague, Cristian Coriano-Ortiz ... Mary J Dunlop
    The LOVdeg tag is a versatile tool for bacterial optogenetics, offering modular, blue light-inducible protein degradation for a range of synthetic biology and metabolic engineering applications.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3

    Tomoki Kita, Kyoko Chiba ... Shinsuke Niwa
    Comparative biochemical analysis of a nematode-specific kinesin-3 shed light on the mechanism of kinesin-3 dimerization and activation.
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Integrating analog and digital modes of gene expression at Arabidopsis FLC

    Rea L Antoniou-Kourounioti, Anis Meschichi ... Martin Howard
    The regulation of gene expression can be either binary (on/off digital regulation) or graded (analog regulation) and these fundamentally different regulatory modes can be integrated to control the expression of the same gene.
    1. Cell Biology

    Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

    Eike K Mahlandt, Sebastián Palacios Martínez ... Joachim Goedhart
    Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.
    1. Genetics and Genomics
    2. Neuroscience

    Single-cell transcriptome profiles of Drosophila fruitless-expressing neurons from both sexes

    Colleen M Palmateer, Catherina Artikis ... Michelle N Arbeitman
    A single-cell RNA-seq study examining gene expression profiles in neurons that underlie Drosophila reproductive behaviors shows males and females build sex-specific behaviors at the molecular-genetic level by overlaying sex-specific information on core gene expression networks.
    1. Immunology and Inflammation
    2. Physics of Living Systems

    Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation

    Derek M Britain, Jason P Town, Orion David Weiner
    A light-controllable ligand is used to probe where in the signaling cascade T cells discriminate self from non-self.
    1. Cell Biology
    2. Genetics and Genomics

    Circadian oscillations in Trichoderma atroviride and the role of core clock components in secondary metabolism, development, and mycoparasitism against the phytopathogen Botrytis cinerea

    Marlene Henríquez-Urrutia, Rebecca Spanner ... Luis F Larrondo
    The biocontrol fungus Trichoderma atroviride possess a functional circadian clock, whose components impact the production of secondary metabolites and also play a role in the interaction with fungi such as the phytopathogen Botrytis cinerea.
    1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    A nucleation barrier spring-loads the CBM signalosome for binary activation

    Alejandro Rodriguez Gama, Tayla Miller ... Randal Halfmann
    Switches in cell state downstream of pathogen exposure are driven by a deeply conserved disorder-to-order phase transition in an immunity signaling network.
    1. Microbiology and Infectious Disease

    Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution

    Daisuke Nakane, Gen Enomoto ... Takayuki Nishizaka
    Colour-controlled c-di-GMP signalling mediates directional switch of phototaxis via within-a-pole regulation of type IV pili in rod-shaped cyanobacteria Thermosynechococcus vulcanus.

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