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    1. Biochemistry and Chemical Biology
    2. Genetics and Genomics

    A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch

    Hélène Duplus-Bottin, Martin Spichty ... Gaël Yvert
    A single protein catalyzes site-specific DNA recombination upon blue-light illumination.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3

    Tomoki Kita, Kyoko Chiba ... Shinsuke Niwa
    Comparative biochemical analysis of a nematode-specific kinesin-3 shed light on the mechanism of kinesin-3 dimerization and activation.
    1. Physics of Living Systems

    Hybrid protein assembly-histone modification mechanism for PRC2-based epigenetic switching and memory

    Cecilia Lövkvist, Pawel Mikulski ... Martin Howard
    Extra protein memory storage elements with positive feedbacks, in addition to histone modifications, can explain epigenetic switching and memory at the PRC2 target gene FLOWERING LOCUS C (FLC).
    1. Cell Biology
    2. Developmental Biology

    Physically asymmetric division of the C. elegans zygote ensures invariably successful embryogenesis

    Radek Jankele, Rob Jelier, Pierre Gönczy
    Systematic analysis of C. elegans zygotes manipulated to divide equally demonstrates that daughter cell size asymmetry is critical for proper cell cycle timing, positioning, and fates during subsequent embryogenesis.
    1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    A nucleation barrier spring-loads the CBM signalosome for binary activation

    Alejandro Rodriguez Gama, Tayla Miller ... Randal Halfmann
    Switches in cell state downstream of pathogen exposure are driven by a deeply conserved disorder-to-order phase transition in an immunity signaling network.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Light-inducible protein degradation in E. coli with the LOVdeg tag

    Nathan Tague, Cristian Coriano-Ortiz ... Mary J Dunlop
    The LOVdeg tag is a versatile tool for bacterial optogenetics, offering modular, blue light-inducible protein degradation for a range of synthetic biology and metabolic engineering applications.
    1. Plant Biology

    Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

    Ivan Kulich, Julia Schmid ... Jiří Friml
    Negative Gravitropic Response of roots (NGRs), pivotal for root gravitropic bending, are indispensable for the gravity-induced translocation of D6 protein kinase, a key regulator of PIN3 auxin efflux carrier activity.
    1. Cell Biology
    2. Developmental Biology

    Optogenetic investigation of BMP target gene expression diversity

    Katherine W Rogers, Mohammad ElGamacy ... Patrick Müller
    Optogenetic manipulation of BMP signaling indicates that diversity in BMP-dependent gene expression is not well explained by differential responses to BMP, and combinatorial signaling is a major driver of diversity.
    1. Cell Biology

    Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

    Eike K Mahlandt, Sebastián Palacios Martínez ... Joachim Goedhart
    Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.
    1. Cell Biology

    Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization

    Kristen Witte, Devin Strickland, Michael Glotzer
    Optogenetic control of Cdc42 activation during polarization of budding yeast demonstrates a cell cycle regulated switch between two distinct modes of positive feedback.