Although complete mouse in vitro spermatogenesis can be achieved, failure of adult Leydig cell development, with accumulation of progesterone and estradiol, androstenedione deficiency, deregulation of steroidogenic, and steroid target gene expression, were highlighted in organotypic cultures, even with hCG.

Exploiting the power of the Fshr reporter, the first comprehensive genetic record of the spatial distribution of FSHR expression offers prospects for extensive exploration of FSH-FSHR biology.

Single-cell chromatin accessibility analysis reveals the intricate regulatory landscape of mouse testicular development, uncovering novel cell subpopulations and transcription factors, and offering valuable insights into the molecular mechanisms driving germ cell and somatic cell maturation.

Gender-affirming hormone therapy in trans women leads to a loss of mature characteristics in the spermatogonial stem cell niche, causes spermatogenic arrest, and triggers fibrotic processes.

For the first time, action potentials and subthreshold postsynaptic potentials of almost all individual identifiable neurons within a functional unit of the leech nervous system were simultaneously imaged during sensory processing and behavioral generation.

Necroptosis triggers organ-specific, age-associated deterioration of male reproductive system.

Mitochondrial functional and structural defects caused by PARL deficiency lead to arrested spermatogenesis and germ cell ferroptosis.

In vitro reprogramming of human fibroblasts to Sertoli-like cells with immunosuppressive properties provides platforms for basic research and clinical application.

The vertebrate testis-determining gene, Sox9, also controls adult testis maintenance in mammals, acting redundantly with its paralog Sox8.