The pathogenic bacterium Listeria monocytogenes spreads infection using a two-tiered strategy, where most bacteria spread locally but a few 'pioneers' move further, increasing the likelihood of a persistent infection.
Combination of stem cell engineering and CRISPR technologies created a facile method to genetically manipulate macrophages, a multifunctional cell type that plays critical roles in immunity, cancer, and tissue homeostasis.
Type I interferon and interferon-γ signaling redundantly protects mice from the tick-borne pathogen Rickettsia parkeri in the skin, and interferon receptor-deficient mice are a tractable model for investigating rickettsiosis.
Activated macrophages initiate a robust DNA damage response that depends on type I IFN and regulates their genetic program and inflammasome activation, establishing a mechanistic link between DNA damage responses and innate immunity.
Statistical analysis and LASSO regression modeling provide insights into pathogen-specific host response patterns in cerebrospinal fluid from different disease etiologies to support future classification of pathogen type based on host response patterns in meningitis.