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    1. Cell Biology
    2. Plant Biology

    Stretch-activated ion channels identified in the touch-sensitive structures of carnivorous Droseraceae plants

    Carl Procko, Swetha Murthy ... Joanne Chory
    Identification of putative mechanisms for mechanosensation-dependent prey capture in carnivorous plants.
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    Synthetic reconstruction of the hunchback promoter specifies the role of Bicoid, Zelda and Hunchback in the dynamics of its transcription

    Gonçalo Fernandes, Huy Tran ... Nathalie Dostatni
    The hunchback transcription features are compatible with an equilibrium model with a short decay length Bicoid activity gradient as the sole source of positional information, while Zelda and Hunchback speed-up the process by different means.
    1. Chromosomes and Gene Expression

    Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

    Jason C Bell, David Jukam ... Aaron F Straight
    ChAR-seq is a massively parallelized de novo RNA mapping assay, which is capable of generating hundreds to thousands of RNA-binding maps with no a priori knowledge of target RNAs.
    1. Computational and Systems Biology

    Dynamic control of gene regulatory logic by seemingly redundant transcription factors

    Zohreh AkhavanAghdam, Joydeb Sinha ... Nan Hao
    Seemingly redundant homologous transcription factors play distinct and cooperative roles in time-dependent combinatorial gene regulation and enable dynamic control of heterogeneity in the gene responses to environmental stresses.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair

    Adi Amar-Schwartz, Vered Ben Hur ... Rotem Karni
    Unbiased proteomics and molecular analysis revealed a new role for S6K1 in regulating DNA repair through the orchestrated phosphorylation of CDK1 and MSH6. The findings may explain why RPS6KB1 gene amplification contributed to breast cancer drug resistance.
    1. Chromosomes and Gene Expression

    The role of RNA in the maintenance of chromatin domains as revealed by antibody-mediated proximity labelling coupled to mass spectrometry

    Rupam Choudhury, Anuroop Venkateswaran Venkatasubramani ... Axel Imhof
    Proximity biotinylation of the Drosophila centromere suggests a role of RNA helicases, centromeric transcripts, and centromeric R-loops for centromere function.
    1. Genetics and Genomics

    Sex-specific splicing occurs genome-wide during early Drosophila embryogenesis

    Mukulika Ray, Ashley Mae Conard ... Erica Larschan
    Combining computational and experimental approaches reveals that the loss of a maternal transcription factor influences sex-biased differential splicing in the early zygotic transcriptome at genes that are critical for normal developmental processes.
    1. Biochemistry and Chemical Biology

    RNA-dependent RNA targeting by CRISPR-Cas9

    Steven C Strutt, Rachel M Torrez ... Jennifer A Doudna
    Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.
    1. Cell Biology

    Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states

    Andrew C Bergen, Rachel A Kocik ... Audrey P Gasch
    Counterintuitively, activation of the transcriptional repressor of growth-promoting genes is important for yeast cell acclimation to salt stress, such that cells with larger activation of the repressor tend to have faster growth acclimation after stress.
    1. Microbiology and Infectious Disease

    Natural mismatch repair mutations mediate phenotypic diversity and drug resistance in Cryptococcus deuterogattii

    R Blake Billmyre, Shelly Applen Clancey, Joseph Heitman
    Eukaryotic pathogens, like Cryptococcus deuterogattii, can use elevated mutation rates to more rapidly adapt to stresses, such as drug challenges, but at the cost of lower fitness in less stressful environments.