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498 results found
    1. Chromosomes and Gene Expression

    MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion

    Ge Zheng et al.
    Systematic analyses of DNA replication machinery components in human cells reveal a requirement of MCM-dependent de novo loading or mobilization of cohesin at replication forks in establishing sister-chromatid cohesion.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding

    Kanokwan Champasa et al.
    Analysis of an essential motif in Mcm4 provides insights into replicative helicase double-hexamer formation and the first step requiring this intermediate during replication initiation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

    Alessandro Costa et al.
    The Mcm2-7 motor unwinds DNA using an approach distinct from that of superfamily III helicases, and accesses multiple ring configurations and assembly states during the initiation of DNA replication.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7

    Lorraine De Jesús-Kim et al.
    A multi-step process of helicase activation sensitizes replication initiation to the extent of replicative helicase phosphorylation.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Antagonistic control of DDK binding to licensed replication origins by Mcm2 and Rad53

    Syafiq Abd Wahab, Dirk Remus
    The mechanism of Dbf4-dependent kinase (DDK) recruitment to replication origins and its regulation by the checkpoint have been identified.
    1. Chromosomes and Gene Expression

    Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones

    Nina Kirstein et al.
    Replication origins are established throughout the genome with the exception of transcribed genes, and the local chromatin composition likely modulates the density of ORC and MCM as well as origin activation.
    1. Chromosomes and Gene Expression

    Multiple kinases inhibit origin licensing and helicase activation to ensure reductive cell division during meiosis

    David V Phizicky et al.
    Meiotic cells inhibit two distinct steps of replisome assembly with multiple kinases to prevent DNA replication between Meiosis I and Meiosis II.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A conserved MCM single-stranded DNA binding element is essential for replication initiation

    Clifford A Froelich et al.
    The crystal structure of the MCM helicase bound to single-stranded DNA reveals a binding motif that is critical for cell viability, helicase activation and DNA replication.
    1. Chromosomes and Gene Expression

    The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure

    Hsiang-Chen Chou et al.
    The initiation of human genome replication requires the six-subunit origin recognition complex (ORC) and CDC6, with ORC playing additional roles during mitosis and in organization of the cell nucleus.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation

    Franziska Bleichert et al.
    Electron microscopy uncovers the structure of the origin recognition complex (ORC) in metazoans, and reveals how mutations in the ORC6 subunit lead to Meier-Gorlin syndrome in humans.

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