Genome editing to insert a light-sensitive dimerization module directly into the EB1 microtubule plus end adaptor gene in human induced pluripotent stem cells enables local and acute optogenetic EB1 inactivation highlighting microtubule functions in developing neuronal growth cones.
Dina Simkin, Kelly A Marshall ... Evangelos Kiskinis
An inducedpluripotent stem cell (iPSC)-based model of KCNQ2-associated developmental epileptic encephalopathy suggests that disease is driven by dyshomeostaic neuronal mechanisms that are downstream of loss of M-current.
Kerstin Hasenpusch-Theil, Christine Laclef ... Thomas Theil
Primary-cilia-mediated processing of the Gli3 transcription factor enables the formation of subtypes of projection neurons in appropriate numbers during the development of the cerebral cortex.
Repressing astroglial PTBP1 by shRNA or antisense oligonucleotide fails to convert astrocyte to neuron including dopaminergic neuron in a 6-OHDA mouse model of PD.
James Alexander Briggs, Victor C Li ... Marc W Kirschner
Single cell RNA sequencing reveals that mouse embryonic stem cells can be differentiated into the same terminal motor neuron state via distinct differentiation paths, one of which includes a surprising intermediate state not found in embryos.
OptoGranules reveal the function of G3BP1 as a stress granule scaffold and demonstrate that protracted stress granule assembly is sufficient to drive neurodegeneration and the evolution of ALS-FTD pathology.
Constitutive deficiency of AP2γ transcription factor impairs hippocampal glutamatergic neurogenesis and induces alterations on limbic-cortical connectivity that contribute to anxiety-like behavior and cumulative memory deficits in mice from juvenile to adult periods.
