Identification of a transporter complex comprised of SLC37A3 and ATRAID is required for nitrogen-containing bisphosphonates to enter the cytosol from lysosomes and exert their therapeutic effects on bone.
Erika A Riederer, Paul J Focke ... Francis I Valiyaveetil
Methodology for the in vitro assembly of multimeric membrane proteins and the utility of the approach for generating heteromeric variants of homo-multimeric proteins is described.
Carlos Manlio Díaz-García, Dylan J Meyer ... Gary Yellen
When neurons are stimulated, calcium entry into mitochondria upregulates mitochondrial energy production, but glycolytic energy production in the cytosol is stimulated by elevated energy demand, not Ca2+ signaling.
Randy B Stockbridge, Janice L Robertson ... Christopher Miller
Efflux of xenobiotic fluoride from microorganisms occurs through a novel family of ion channels with stringent selectivity for fluoride ion and dual-topology molecular architecture.
Marian Breuer, Tyler M Earnest ... Zaida Luthey-Schulten
A near-complete flux balance analysis model of a minimal cell demonstrates the high essentiality of its metabolic genes, agrees well with experimental essentiality data and suggests some further gene removals.
Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.
Combined simulations and electrophysiological experiments show that the CLC channels and exchangers form physically distinct and evolutionarily conserved pathways through which Cl- and H+ ions move when crossing biological membranes.
Cryo-EM structure and functional analyses show that human iron exporter ferroportin (Fpn) can bind and transport calcium ions and the transport activity is reduced when the concentration of ferrous ions is high.
Crystallography together with electron-resonance spectroscopy, molecular-dynamics simulations, and transport measurements reveal the molecular details of protein conformational change, and how this change contributes to function in a CLC-type chloride/proton exchanger.
