Long noncoding RNA Norad performs an essential function in mammalian physiology by inhibiting the activity of PUMILIO RNA binding proteins, thereby suppressing a multi-system degenerative phenotype resembling premature aging.
Promoter capture Hi-C in human iPSCs and iPSC-derived cardiomyocytes provides a platform to interrogate gene-regulatory dynamics of cardiomyocyte differentiation and directly links thousands of cardiovascular disease risk loci to hundreds of distal target genes.
Certain types of 3D chromatin loops are easy to predict from existing or easily obtainable 2D information, which benefits gene expression studies in tissues/cells/organisms without extensive pre-existing 3D information.
Promoter interactome maps in human embryonic stem cells (ESCs) and ESC-derived early neuroectodermal progenitors link distal enhancers to putative target genes, reveal lineage-specific cis-regulatory architecture and shed light on the logic of gene regulation by multiple enhancers.
Deep penetration and transmission of mechanical force to regulate ER functions depends on not only the passive cytoskeletal support, but also the active actomyosin contractility, which is dispensable for mechanotransduction at the plasma membrane.