The turquoise killifish from ephemeral pools in African savannah combines extremely short lifespan with a standard vertebrate body plan – ideal attributes for a laboratory animal.
David Willemsen, Rongfeng Cui ... Dario Riccardo Valenzano
Population genetics in turquoise killifish wild populations reveals how small population size and genetic drift determine the accumulation of deleterious gene variants leading to short lifespan.
Patrick Smith, David Willemsen ... Dario Riccardo Valenzano
Resetting a young gut microbiota in middle-aged individuals extends life span and slows aging in the naturally short-lived turquoise killifish, a new vertebrate model organism to study how the microbiota affects the aging process.
Johannes Krug, Birgit Perner ... Christoph Englert
Using the short-lived turquoise killifish, a transparent in vivo reporter line named klara is established that will be a valuable tool to explore development, senescence, aging, and regeneration.
William John Bradshaw, Michael Poeschla ... Dario Riccardo Valenzano
Naturally short-lived turquoise killifish undergo systemic and mucosal spontaneous decline in antibody-repertoire diversity during aging, recapitulating in a few months the aging-dependent decline of adaptive immunity.
Rapid and efficient CRISPR/Cas9-mediated knock-in of fluorescent reporters at various genomic loci enables cell- and tissue-specific expression and establishes the short-lived African killifish as a vertebrate system for precise genetic engineering at scale.
An automated feeding system to precisely control feeding in the killifish opens new areas to explore vertebrate lifespan, cognitive decline, dietary interventions, and drug screening in a high-throughput manner.
A novel in vivo strategy for reversible, multiplexed control of circulating hormone expression levels, achieved by combining genome-editing and intramuscular electroporation of fluorescently tagged hormones in the naturally short-lived turquoise killifish.
