OCT4 and SOX2 display partially independent activity to regulate chromatin accessibility, and highly dynamic activity of OCT4 is required throughout the cell cycle to maintain pluripotency enhancer accessibility.
A previously unrecognized transcriptional coactivator function of the dyskerin ribonucleoprotein complex and its associated small nucleolar RNA has been uncovered and mediates embryonic stem cell-specific transcription.
In contrast to other transcription factors, CTCF and Esrrb rapidly regain binding after replication and remain bound to their targets during mitosis, preserving local nucleosome organization throughout the cell cycle.
Post-implantation epiblast maturation and patterning of anterior-posterior axis in mouse embryonic development are mediated by pluripotency transcription factor Zfp281 through transcriptional and epigenetic control of Nodal signaling.
The chromatin remodeller BRG1 is recruited to pluripotency-associated gene regulatory elements by the pioneer transcription factor OCT4 to support further transcription factor binding and gene regulation.