The conserved polypeptide transport associated (POTRA) domains of the protein import channel, Toc75, are essential for protein import into chloroplasts.

Phylogenomics provides support for the Gram-positive type of bacterial cell envelope being a derived character that arose independently multiple times through loss of an ancestral outer membrane.

One minute biotinylation with APEX2 peroxidase in living cells identifies established and new components of mitochondrial and ER membranes; dataset intersection and overexpression screen identifies SYNJ2BP overexpression as inducing mitochondria-rough ER contacts.

An easy-to-implement antibody validation pipeline addresses the reproducibility crisis resulting from the use of non-specific antibodies.

Outer membrane β-barrel proteins in Gram-negative bacteria are assembled within the lumen of the BamA β-barrel.

Temperature-sensitive mitochondrial outer membrane proteins used as novel quality control substrates reveal a unique mitochondria-associated degradation pathway consisting of both cytosolic and mitochondrial ubiquitin-proteasome system machinery.

Knockoff is a tool for researchers who wish to acutely disrupt a membrane protein.

Structures of CdiB outer membrane transporters reveal the channel-gating mechanism for the secretion of large bacterial toxins.

eMags is an engineered photodimerizer pair for optogenetic modulation in mammalian cells that is especially suited for the manipulation of intracellular processes occurring in small volumes or subcellular organelles.

In vivo crosslinking approaches revealed that the edge-strand of Escherichia coli metallopeptidase BepA interacts with an assembly intermediate of β-barrel outer membrane protein LptD on the outer membrane protein translocon for its targeting to degradation or maturation.