Functional and mechanistic analysis of p53-regulated lncRNA PINCR and its interacting RNA-binding protein Matrin 3 uncovers context-dependent regulation of specific p53 target genes during DNA damage.
Cryo-EM structures of actomyosin VI in multiple nucleotide states reveal a unique actin-myosin interface and a mechanism of force-sensitivity; furthermore, myosin VI remodels F-actin, suggesting a role for actin structural plasticity during force generation.
Exogenous opioid analgesia is mediated by MORs expressed in glutamatergic neurons, whereas endogenous opioid analgesia is mediated by MORs expressed in GABAergic neurons.
Supporting cells in the cochlea change their shape in response to purinergic receptor activation, which influences hair cell excitability by altering potassium redistribution in the extracellular space.
Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
Mild myelin disruption leads to early axonal pathology, a novel pathological response in neural stem cells, regionally increased oligodendrocytes and altered behavior.
A similar pattern of developmental synaptic refinement leads two consecutive synaptic relays to have the same organization suggesting that some general principle of neural organization is at play.
The reconstitution of a functional envelope protein from an extinct hominid retrovirus reveals its receptor and an ancient host defense that may have led to the extinction of the virus.
A mouse virtual reality system is presented which allows normal spatial behavior and place, grid and head-direction cell firing patterns in 2-D arenas, and is compatible with electrophysiology and multi-photon imaging.
