Computational modelling together with experimental manipulation indicate that the stability and turnover of activated Notch is inextricably linked to the regulation of the pace of segmentation clock gene expression in the presomitic mesoderm.
Operonic mRNAs in bacteria are comprised of ORF (open reading frame)-wide units of secondary structure, which are intrinsically distinct between adjacent ORFs and encode a rough blueprint for ORF-specific translation efficiency.
A mouse virtual reality system is presented which allows normal spatial behavior and place, grid and head-direction cell firing patterns in 2-D arenas, and is compatible with electrophysiology and multi-photon imaging.
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
Cryo-electron microscopy reconstructions of two microtubule-bound transport kinesins at 7 Å resolution reveal how microtubule track binding stimulates ADP release, primes the active site for ATP binding and enables force generation.