The polyQ tract of pathogenic Huntingtin causes aggregation when expanded in Huntington’s disease, but its two flanking domains control its conformational landscape, proteostasis and neurotoxicity.
Theodore J Zwang, Rachel E Bennett ... Bradley T Hyman
Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.
Atefeh Rafiei, Sofía Cruz Tetlalmatzi ... David C Schriemer
Doublecortin activates its C-terminal domain when its N-terminal domain engages the microtubule lattice, leading to self-associations that explain the stabilizing effect of Doublecortin in neuronal migration.
Lab-evolved 'super Spy' chaperones show enhanced flexibility, which allows them to bind to and stabilize proteins more effectively than natural chaperones.
Gemma LM Fisher, César L Pastrana ... Mark S Dillingham
A combination of structural, biochemical, single-molecule and in vivo methods are used to show how ParB locally condenses the bacterial chromosome near the origin and earmarks this region for segregation.
Hitendra Negi, Aravind Ravichandran ... Ranabir Das
Biophysical experiments, pulse-chase assays, and molecular dynamics simulations reveal how the proteasome targeting signal Fat10 can modulate the structure of substrate proteins to potentially regulate their degradation by the proteasome.
Dennis V Pedersen, Martin Nors Pedersen ... Gregers R Andersen
Combining electron microscopy and solution scattering demonstrated that properdin oligomers adopt extended rigid and well-defined conformations that are integral to the biological function of this complement regulator.
Shunshi Kohyama, Natsuhiko Yoshinaga ... Nobuhide Doi
A cell-sized fully confined space significantly controls the emergence and stability of a protein wave, resulting in intracellular spatiotemporal regulation driven by a reaction-diffusion mechanism.
Liquid-liquid phase separation of tau is demonstrated to be an equilibrium state, stable only within a narrow range near physiological conditions, and thus has the capacity to regulate biological processes.
