Co-evolving residue pairs in the different components of a protein complex almost always make contact across the protein–protein interface, thus providing powerful restraints for the modeling of protein complexes.

A combination of phylogenetic and biochemical analyses suggest that some unexpected variations in the synthesis of isoprenoids may be widespread across all three domains of life.

The amination network for the distribution of assimilated ammonium throughout metabolism is defined by promiscuous transaminases, allowing a high flexibility of the network that supported engineering novel connections and even alternative ammonium assimilation entry points.

During growth in biofilms and host infection, the opportunistic pathogen Pseudomonas aeruginosa employs respiratory heterocomplexes that support extracellular electron shuttling and pathogenicity.

Structures of CysZ show a antiparallel membrane protein with an unanticipated fold and together with functional characterization provide insight into a bacterial sulfate translocating system.