1,601 results found
    1. Structural Biology and Molecular Biophysics

    Structural mechanism of ATP-independent transcription initiation by RNA polymerase I

    Yan Han et al.
    Structures of RNA polymerase I transcription machinery revealed a ratcheting motion within the complex in coordination with three distinct functional states, implicating a novel mechanism for promoter bubble opening in the absence of ATP hydrolysis.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    The cryo-EM structure of a 12-subunit variant of RNA polymerase I reveals dissociation of the A49-A34.5 heterodimer and rearrangement of subunit A12.2

    Lucas Tafur et al.
    Cryo-EM structures of RNA polymerase I reveal considerable 'transformers-like' rearrangements where one subcomplex dissociates and is replaced by one domain of another subunit, possibly as an additional layer of transcriptional control.
    1. Chromosomes and Gene Expression

    The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription

    Eva Torreira et al.
    Live-cell imaging, genetic analysis and electron cryomicroscopy identify structural motifs involved in the differential assembly of Pol I-Rrn3 complexes and Pol I homodimers in response to nutrient availability.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex

    Jin Young Kang et al.
    Cryo-electron microscopy structures show how coliphage HK022 Nun blocks Escherichia coli RNA polymerase translocation by mediating multiple interactions between the RNA polymerase and nucleic acids.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    E. coli TraR allosterically regulates transcription initiation by altering RNA polymerase conformation

    James Chen et al.
    Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
    1. Chromosomes and Gene Expression

    Resection is responsible for loss of transcription around a double-strand break in Saccharomyces cerevisiae

    Nicola Manfrini et al.
    The conversion of the ends of a double-strand break from double-stranded to single-stranded DNA, which is necessary to initiate homologous recombination, is responsible for loss of transcription and RNA polymerase occupancy around the double-strand break in Saccharomyces cerevisiae.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    GE23077 binds to the RNA polymerase ‘i’ and ‘i+1’ sites and prevents the binding of initiating nucleotides

    Yu Zhang et al.
    The cyclic-peptide antibiotic GE23077 inhibits bacterial RNA polymerase through a novel target that exhibits low susceptibility to target-based resistance and that enables synthesis of bipartite inhibitors that are exceptionally potent and refractory to target-based resistance.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    A global change in RNA polymerase II pausing during the Drosophila midblastula transition

    Kai Chen et al.
    The first zygotic genes are transcribed without RNA polymerase II pausing from a TATA-enriched promoter.
    1. Structural Biology and Molecular Biophysics

    cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ

    Rafael Fernandez-Leiro et al.
    Structures of the replicative DNA polymerase Pol IIIα, the DNA sliding clamp, the proofreading exonuclease, and the processivity switch Tau (τ) suggest a mechanism for quick release during lagging strand synthesis.
    1. Structural Biology and Molecular Biophysics

    Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts

    Hande Boyaci et al.
    Cryo-electron microscopy structures show how the clinically used antimicrobial fidaxomicin binds and inhibits Mycobacterium tuberculosis RNA polymerase by acting like a doorstop to jam the enzyme in an open conformation via the general transcription factor RbpA.

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