RNA polymerase II generates numerous transcript isoforms, including transcripts initiating downstream of the START codon, that are efficiently degraded by the nonsense-mediated mRNA decay pathway.

Fundamental details of the rate and molecular spectrum of transcript errors were revealed in four bacterial species, providing novel insights into transcriptional fidelity and RNA quality-control in prokaryotes.

p63 uses an intricate network of domain-domain interactions to control its function in long-term quality maintenance of resting oocytes.

An eukaryotic specific system cooperates with the ribosome quality control pathway to maintain the reading frame during translation of slowly translated sequences.

Protein aggregates resulting from mutations in C9orf72 impair different aspects of cellular quality control in the cytosol and the nucleus, but mRNA-mediated effects contribute more strongly to toxicity.

Endothelial Differentiation Factor 1 (EDF1) plays a critical role in driving mRNA-specific quality control and global transcriptional responses in response to ribosome collisions.

Alkylation stress modify mRNAs and results in translation arrest, which activates the ribosome-rescue pathway of trans translation in bacteria.

Yeast extracts recapitulate a quality-control pathway dedicated to rescuing stalled ribosomes, providing unexpected insights into a noncanonical elongation reaction and the fate of incompletely synthesized proteins.

A hierarchical relationship among highly conserved regulatory ribosomal ubiquitylation events and the presence of antagonistic deubiquitylating enzymes suggests a dynamic ubiquitin code impacts ribosome-associated activities.

Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.