Affinity capture of polyribosomes followed by RNAseq(ACAPSeq) is a technique that harnesses massively parallel sequencing to identify protein-protein interactions from any source from which polyribosomes can be purified.
Live-cell nanometer-resolution RNA labeling method enables transcriptome-wide mapping of endogenous RNAs in nuclear, cytosol, ER, and mitochondrial subcompartments.
RNAs enriched at cell protrusions are translated regardless of their location in the cytoplasm but are dynamically repressed in retracting protrusions and incorporated into heterogeneous RNA clusters.
Defining the biological functions of long non-coding RNAs, individually or as a class, and teasing apart the role of underlying genomic sequences remains the biggest challenge for this field.
In Caenorhabditis elegans histone methylation (H3K9me3) controls the synthesis of heritable small RNAs in a gene-specific manner and thus enables 'flagging' of newly-acquired genes.
Superfamilies of trans-species small RNAs from the parasitic plant Cuscuta have sequence variation that correspond to synonymous site variation in host plant target mRNAs.
Neuronal ELAV-like (nELAVL) proteins are associated with non-coding Y RNAs in stressed neurons and in the brains of Alzheimer's disease patients, suggesting a new means of regulatory protein sequestration and mRNA target regulation.
Computational analysis of MNase-seq data reveals that alternatively positioned nucleosomes are prevalent in both yeast and human cells and create significant heterogeneity within cell populations.
