1,759 results found
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    Gene autoregulation by 3’ UTR-derived bacterial small RNAs

    Mona Hoyos, Michaela Huber ... Kai Papenfort
    Bacterial small RNAs derived from the 3' untranslated region of an mRNA can serve as autoregulatory elements.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome

    An Van den Bossche, Steven W Hardwick ... Rob Lavigne
    A bacteriophage remodels the RNA metabolism machinery of a bacterial host to sequentially clear host transcripts and then stabilize the viral RNA.
    1. Microbiology and Infectious Disease

    Glutamine synthetase mRNA releases sRNA from its 3′UTR to regulate carbon/nitrogen metabolic balance in Enterobacteriaceae

    Masatoshi Miyakoshi, Teppei Morita ... Kan Tanaka
    Under nitrogen limitation, Salmonella and Escherichia coli express a messenger RNA to translate glutamine synthetase, and concomitantly the messenger RNA produces a small RNA from its 3′ end to inhibit the translation of 2-oxoglutarate dehydrogenase of the TCA cycle.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells

    Seongjin Park, Karine Prévost ... Jingyi Fei
    RNA chaperone Hfq dynamically binds various pools of cellular RNAs to execute different regulatory functions.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist

    Sarah Lauren Svensson, Cynthia Mira Sharma
    Functional characterization of a pair of cis-encoded antisense RNAs reveals a role for RNase III and antisense regulation in the biogenesis and regulatory activity of a bacterial virulence factor-regulating small RNA.
    1. Cell Biology

    Spatial organization shapes the turnover of a bacterial transcriptome

    Jeffrey R Moffitt, Shristi Pandey ... Xiaowei Zhuang
    Transcriptome-scale RNA imaging and lifetime measurements reveal that the E. coli transcriptome is spatially organized and that this organization modulates the post-transcriptional fate of bacterial mRNAs.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Tracing a protein’s folding pathway over evolutionary time using ancestral sequence reconstruction and hydrogen exchange

    Shion An Lim, Eric Richard Bolin, Susan Marqusee
    Pulsed-labeling hydrogen exchange on the ribonuclease H family show that the major folding intermediate is conserved over three billion years of evolution, but the path leading to this intermediate varies.
    1. Biochemistry and Chemical Biology

    Protomer alignment modulates specificity of RNA substrate recognition by Ire1

    Weihan Li, Kelly Crotty ... Peter Walter
    Biochemical analysis and computational modeling reveal how cells mechanistically control the quality of their proteomes and demonstrate that the precise alignment of subunits in oligomeric complexes can profoundly affect enzymatic properties.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA

    Hemant N Goswami, Jay Rai ... Hong Li
    Three-dimensional structure of CRISPR Cas7-11 shed light on protein evolution and guide development of biotechnology for RNA manipulations in cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli

    Dipali G Sashital, Candacia A Greeman ... James R Williamson
    Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.

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