A combination of animal models reveal how the molecular mechanisms of exosome secretion (RalA/B-dependent) are linked to their cargo content and their function in breast cancer pre-metastatic niche formation.
David J Forsthoefel, Nicholas I Cejda ... Phillip A Newmark
Application of laser-capture microdissection to planarian intestinal tissue provides a new tool for analysis of tissue-specific gene expression in flatworms, and a new resource to advance investigations of gastrointestinal regeneration.
Enteroendocrine cells sense nutrients in the gut and regulate digestive physiology but are rendered insensitive following fat ingestion due to alteration of gut microbiota.
Alison K Gillingham, Jessie Bertram ... Sean Munro
Relocation of Rab, Ras and Rho family GTPases to the surface of mitochondria enables efficient identification of their effectors, exchange factors and GAPs by proximity biotinylation.
By moving from correlations to causality in cancer signal transduction using optogenetics, the sufficiency of RalB activation to trigger invasion and the underlying molecular mechanisms were established.
The symbiotic relationship between Hydra and Chlorella is driven by metabolic co-dependence and characterized by changes in the photobiont genome in terms of lack of genes essential in free-living algae.
A new post-translational regulatory mechanism of K-Ras is identified, which expands the function of reversible protein lysine fatty acylation and offers new possibility to target the K-Ras oncoprotein.
Arman Javadi, Ravi K Deevi ... Frederick C Campbell
PTEN organizes multicellular architecture by non-catalytic scaffolding of spatially localized β-Arrestin1/ARHGAP21/Cdc42 protein complexes to control mitotic spindle orientation, multicellular configuration and lumen formation.
Sequence conservation in Ras depends strongly on the biochemical network in which it operates, providing a framework for understanding the origin of global selection pressures on proteins.
Filamin, by recruiting a signaling complex to the postsynapse, orchestrates the correct formation and growth of postsynaptic membranes and localization of glutamate receptor subunits.
