A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.
Tarah Elizabeth Trebino, Borna Markusic ... Zhihong Wang
An in-depth binding profile analysis of BRAF domains related to RAF activation and autoinhibition unveils the distinctive roles of each domain in selecting preferred RAS isoforms and facilitating autoinhibition.
In the injured sciatic nerve, blood-derived monocytes and macrophages eat dying leukocytes, thereby contributing to nerve debridement and inflammation resolution, and this correlates with neuronal regeneration.
Marian Breuer, Tyler M Earnest ... Zaida Luthey-Schulten
A near-complete flux balance analysis model of a minimal cell demonstrates the high essentiality of its metabolic genes, agrees well with experimental essentiality data and suggests some further gene removals.
Cryo-EM, Molecular Dynamics, and in vitro binding assays are combined to elucidate a new occluded conformation associated with zinc binding and energy coupling with the proton motive force.
Computational methods based on both sequence and structure show evidence that tyrosine kinases are energetically biased to populate the 'DFG-out' type-II inhibitor binding conformation relative to serine/threonine kinases.
Bercem Dutagaci, Grzegorz Nawrocki ... Michael Feig
Charge complementarity between RNA and proteins may be a universal principle for phase separation in biology without requiring disorder or specific multivalent interactions.
A screen targeting RNA-associated proteins reveals that PSI regulates timeless alternative splicing and thus controls the period of Drosophila circadian behavior and its phase under temperature cycles.
