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    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution

    Michael Luke Carlson, John William Young ... Franck Duong
    A simple, yet elegant method for robust self-assembly of diverse membrane proteins into soluble peptide nanoparticles for their structural and functional analysis in detergent-free solutions.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Activation of Toll-like receptors nucleates assembly of the MyDDosome signaling hub

    Sarah Louise Latty, Jiro Sakai ... Clare E Bryant
    The strength of Toll-like receptor signalling depends on the number and size of MyDDosomes formed as well as on how quickly these structures assemble.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host

    Francesca D'Angelo, Elena Fernández-Fueyo ... Gregory Bokinsky
    The intracellular network that distributes iron-sulfur clusters in bacteria is surprisingly 'plug and play' with iron-sulfur enzymes acquired from distantly related species, whereas the intracellular electron transfer network often needs plug adapters to connect with foreign enzymes.
    1. Biochemistry and Chemical Biology

    Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks

    Suwen Zhao, Ayano Sakai ... Matthew P Jacobson
    Genome neighborhood networks provide an efficient large-scale approach to mine metabolic pathway context for entire families of proteins and enzymes.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    CRISPR-based functional genomics in human dendritic cells

    Marco Jost, Amy N Jacobson ... Jonathan S Weissman
    A CRISPR-based method enables targeted knockouts and genetic screens in human dendritic cells.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Coupling chemosensory array formation and localization

    Alejandra Alvarado, Andreas Kjær ... Simon Ringgaard
    Formation and cell pole-localization of chemotactic signaling-arrays is a coupled process mediated by ParP, which drives localized array-assembly and regulates the localization-dynamics of its network constituents.
    1. Plant Biology
    2. Computational and Systems Biology

    The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers

    Asako Ishii, Jianyu Shan ... Jun Minagawa
    The structure of the photosystem I (PSI) supercomplex in Ostreococcus tauri has revealed a unique hybrid of both plant-type and green algal-type PSI supercomplexes with three Lhcp trimers located at the ‘state 2’ position.
    1. Structural Biology and Molecular Biophysics

    Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

    Shan Zhou, Weiwei Wang ... Hongri Gong
    The three-dimensional structures of Mycobacterium tuberculosis cytochrome bcc in complex with the antituberculosis agents, Q203 and TB47, explain how these inhibitors suppress activity of the complex.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    Gene autoregulation by 3’ UTR-derived bacterial small RNAs

    Mona Hoyos, Michaela Huber ... Kai Papenfort
    Bacterial small RNAs derived from the 3' untranslated region of an mRNA can serve as autoregulatory elements.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    An updated phylogeny of the Alphaproteobacteria reveals that the parasitic Rickettsiales and Holosporales have independent origins

    Sergio A Muñoz-Gómez, Sebastian Hess ... Andrew J Roger
    Diverse sophisticated phylogenetic analyses update the phylogeny of the Alphaproteobacteria and show that the parasitic Holosporales is a derived group within the Rhodospirillales order which comprises primarily free-living alphaproteobacteria.