The molecular determinants for neuronal subcellular RNA transport by FMRP are defined, with interactions between RNA G-quadruplexes and the RGG domain of FMRP being of critical importance.
SeqZip is a new DNA ligation-based method to condense and maintain exon connectivity information within individual RNA molecules, which can provide new insights into alternative splicing.
Human proteins that add or remove the methyladenosine modification of cellular RNA, or recognize methylated RNA significantly affect HIV-1 infection or viral protein synthesis in cells, suggesting an important role for HIV-1 RNA methylation in regulating viral replication.
In nematode worms, NSUN-1 methylates ribosomal RNA and influences phenotypes related to aging, stress resistance, germ line development, and cuticle integrity by regulating translation of specific mRNAs.
The genome sequence of Trichoplusia ni enables the use of this widespread lepidopteran pest as a model for both the study of small RNA pathways and insecticide resistance.
Cryo-EM structures of the 30S*RNAP complex visualize co-localization of the transcription and translation machineries and provide insights into the transcription-translation synchrony, which coordinates gene expression in bacteria.
In rotaviruses, the selective packaging of eleven distinct genomic RNA segments requires virus-encoded protein NSP2 to alter the RNA structures, facilitating their interactions with each other.
Mapping of 30 general RNA degradation factors onto the yeast transcriptome provides the global distribution of factors for RNA turnover and surveillance in a eukaryotic cell.
Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
