24 results found
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells

    Ananya Mukherjee et al.
    Unique biosensor design and protein-engineering enables direct visualization of the active form of Fyn kinase with high specificity, minimal perturbation and shows cellular signaling to be compartmentalized and pulsatile.
    1. Biochemistry and Chemical Biology

    The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling

    Januka S Athukoralage et al.
    The dynamic interplay between type III CRISPR enzymes governs cyclic nucleotide levels and infection outcomes in virus-host conflict.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The guide sRNA sequence determines the activity level of box C/D RNPs

    Andrea Graziadei et al.
    Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A conserved MCM single-stranded DNA binding element is essential for replication initiation

    Clifford A Froelich et al.
    The crystal structure of the MCM helicase bound to single-stranded DNA reveals a binding motif that is critical for cell viability, helicase activation and DNA replication.
    1. Cell Biology

    Spatial organization shapes the turnover of a bacterial transcriptome

    Jeffrey R Moffitt et al.
    Transcriptome-scale RNA imaging and lifetime measurements reveal that the E. coli transcriptome is spatially organized and that this organization modulates the post-transcriptional fate of bacterial mRNAs.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Archaeal TFEα/β is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39

    Fabian Blombach et al.
    An archaeal basal transcription factor containing an iron-sulphur cluster sheds light on the evolution of transcription machineries in archaea and eukaryotes.
    1. Structural Biology and Molecular Biophysics

    Crystal structure of the full Swi2/Snf2 remodeler Mot1 in the resting state

    Agata Butryn et al.
    The structure of the entire Mot1 Swi2/Snf2 protein uncovers an unexpected auto-inhibited resting state activated by substrate binding, and suggests a DNA pulling mechanism of TBP displacement.
    1. Biochemistry and Chemical Biology

    Control of cyclic oligoadenylate synthesis in a type III CRISPR system

    Christophe Rouillon et al.
    Generation of the anti-viral second messenger cyclic oligoadenylate by type III CRISPR systems is tightly controlled in response to viral RNA load and sequence.
    1. Structural Biology and Molecular Biophysics

    Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery

    Bertram Daum et al.
    Using both electron cryo-tomography and helical reconstruction, the first structure of the entire archaellum machinery with an assembled filament has been determined, providing the structural basis for our understanding of archaeal motility.
    1. Biochemistry and Chemical Biology

    Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

    Clare Rollie et al.
    The acquisition of new foreign DNA by the prokaryotic adaptive immune system CRISPR-Cas is shown to depend on the fundamental sequence specificity of the Cas1 integrase.

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