Steven Henikoff, Srinivas Ramachandran ... Jorja G Henikoff
A stable tetrameric nucleosome occupies the central segment of each ∼120-bp budding yeast centromere in two rotational phases of both reflectional orientations in vivo.
Internally tagged, functional Cse4/CENP-A/CenH3 histone variant is exclusively centromeric and stable through the budding yeast cell cycle after replacement in S phase.
A non-coding RNA-based targeting mechanism could potentially epigenetically maintain specialized chromatin structures, such as the centromere, in vivo.
Satyakrishna Pentakota, Keda Zhou ... Karolin Luger
A comprehensive structural, biochemical, and cell biological analysis reveals the molecular mechanism and significance of the conserved interaction of centromeric protein N (CENP-N) with the centromeric nucleosome.