A cross-linking screen finds proteins that interact with TANGO1 to export Procollagen VII, and suggests a new model for the secretion of these bulky cargoes that are involved in skin and bone formation.
Imke Ensinck, Alexander Maman ... Folkert J van Werven
The N6-methyladenosine (m6A) methyltransferase complex in budding yeast is highly conserved, yet reconfigured with respect to its mammalian counterpart and has both m6A-dependent and m6A-independent functions.
The structures of Slo1 in complex with b4 imply that the auxiliary beta subunits modulate the channel's gating properties through stabilizing ‘pre-existing’ conformations rather than creating new ones.
Functional and computational studies reveal that the allosteric inhibition mechanism is conserved between glutamate and neutral amino acid transporters of the SLC1 family, and identify a novel allosteric inhibitor.
Braden T Lobingier, Daniel P Nickerson ... Alexey J Merz
Sec1/Munc18 (SM) proteins shield SNARE complexes from NSF/Sec18-mediated disassembly through cooperative binding interactions with SNARE complexes and the universal co-chaperone α-SNAP/Sec17.
SIMC1 and SLF1 bind exclusively to SLF2 to form two separate complexes that direct the human SMC5/6 complex to its antiviral defense or DNA lesion repair activities.
Intersectin counterparts in yeast recruit WASP and WIP to endocytic sites to establish a robust multivalent SH3 domain-PRM interaction network which gives actin assembly onset a switch-like behavior in vivo.
