2,500 results found
    1. Cell Biology

    Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP

    Gaétan Bader, Ludovic Enkler ... Hubert Dominique Becker
    The Bi-Genomic Mitochondrial-Split-GFP, where both fragments of the Split-GFP are expressed by separated translation machineries, shuts off cytosolic fluorescence of dual-localized proteins, allowing visualization of their mitochondrial echoforms.
    1. Neuroscience

    Microtubule-dependent ribosome localization in C. elegans neurons

    Kentaro Noma, Alexandr Goncharov ... Yishi Jin
    The utility of split GFP for tissue-specific visualization of ribosomes in live Caenorhabditis elegans demonstrates the link between ribosomes and microtubules.
    1. Microbiology and Infectious Disease

    Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis

    Eva S Istvan, Sudipta Das ... Daniel E Goldberg
    The malaria parasite Niemann-Pick Type C1-related protein is an important new antimalarial target.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Development and characterization of new tools for detecting poly(ADP-ribose) in vitro and in vivo

    Sridevi Challa, Keun W Ryu ... W Lee Kraus
    Poly(ADP-ribose) (PAR) Trackers (PAR-Ts) are optimized PAR sensors that allow the detection and quantification of PAR levels in extracts, living cells, and living tissues with high sensitivity, and with temporal and spatial precision, in many different experimental and biological systems.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

    Dorottya Anna Simon, András Tálas ... Ervin Welker
    Prime editor activity reporter (PEAR) is a flexible fluorescent reporter that identifies and enriches prime edited cells.
    1. Cell Biology

    Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends

    Samuel J Gonzalez, Julia M Heckel ... Melissa K Gardner
    Computational modeling combined with biophysical and cellular experiments reveal that rapid binding to incomplete, partial microtubule binding sites facilitates tracking of the end-binding protein EB1 with growing microtubule plus-ends.
    1. Cell Biology

    Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

    Congyan Zhang, Randy Schekman
    A human endogenous fusogen, syncytin, which facilitates placental cell-cell fusion during early embryonic development, mediates the formation of open-ended membrane tubular connections between other cell types that efficiently promotes the intercellular transfer of cytoplasmic cargos including Cas9 protein and organelles.
    1. Neuroscience

    New genetic tools for mushroom body output neurons in Drosophila

    Gerald M Rubin, Yoshinori Aso
    Improved cell-type-specific genetic drivers for the study of learning and memory and other complex behaviors in Drosophila are provided and validated.
    1. Plant Biology

    The manifold actions of signaling peptides on subcellular dynamics of a receptor specify stomatal cell fate

    Xingyun Qi, Akira Yoshinari ... Keiko U Torii
    A binary cell fate decision to be or not to be stomata is regulated by multiple peptide ligands, each triggering a unique subcellular dynamics of their shared receptor.

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