Cryo-EM structures of the AAA+ ClpXP protease bound to an ssrA degron reveal the mechanism of substrate recognition and show how the machine transitions from recognition to translocation and unfolding.
RecO efficiently displaces SSB from ssDNA without consuming ATPs using its two DNA-binding sites, even though SSB binds to ssDNA approximately 300 times more strongly than RecO does.
Clear guidelines and open-source software resources are provided for the stop-signal task, in order to streamline the use of this important tool, and to thereby increase its validity and reliability.
Single-molecule force and fluorescence spectroscopy reveal the structural states and dynamics of E. coli single-stranded DNA binding proteins and the energy landscape of the nucleo–protein complex.
The brown planthopper has evolved a highly adaptive oviposition strategy by exploiting caterpillar-induced plant volatiles that provide safe havens for its offspring.