CryoEM reveals the structure of a two-component archaeal S-layer, which sheds new light on archaeal cell biology.

The surface layer of Sulfolobus acidocaldarius consists of a flexible but stable outer protein layer that interacts with an inner, membrane-bound protein.

By using mass spectrometry, we determined the global levels of various DNA modifications across species and tissues.

Prokaryotic and eukaryotic flagella follow a common trend in swimming cost-effectiveness, but eukaryotic flagella are too large for cells with prokaryote volumes, yielding insight into flagellar dissimilarity between taxa.

The crenarchaeon Sulfolobus synthesizes the N-glycan core in the identical way as all Eukaryotes, which strengthens the hypothesis that the eukaryotic N-glycosylation is acquired from an ancient archaeon during eukaryogenesis.

Synechocystis switches its redox pools under reducing photomixotrophic conditions from utilizing NAD(H)- to ferredoxin-dependent enzymes and thereby balances its metabolism in a trade-off between energy conservation and chemical driving forces.

Term-seq, genetic and biochemical experiments reveal that the trans-action factor aCPSF1 and the terminator U-tract cis-element work in a noteworthy two-in-one termination mode in archaea, and may represent an archetypal mode of the eukaryotic RNA polymerase II termination machinery.

The archaeal MCM helicase can load in multiple orientations on DNA but translocation proceeds with a leading N-terminal domain, which affects double hexamer activation at origins of replication.

An investigation into 8-oxodeoxguanosine bypass by archaeal DNA polymerases.