1,160 results found
  1. Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope

    Shotaro Otsuka et al.
    Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
    1. Cell Biology

    In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster

    Sebastian Schnorrenberg et al.
    Building on previous work (Grotjohann et al., 2012), low-light super-resolution microscopy has been performed on living transgenic Drosophila larvae and tissues.
    1. Cell Biology
    2. Neuroscience

    The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction

    Ana Rita Costa et al.
    The axonal cytoskeletal contains an actomyosin-II network that controls circumferential axonal contraction and expansion with the potential of regulating fluctuations in diameter during action potential firing, trafficking, and degeneration.
    1. Microbiology and Infectious Disease

    Nanoscale organization of rotavirus replication machineries

    Yasel Garcés Suárez et al.
    Super-resolution microscopy reveals, at nanometric-scale, the highly organized protein structure of viroplasms, the viral factories used by rotavirus to replicate its genome and assemble new viral particles.
    1. Structural Biology and Molecular Biophysics

    rsEGFP2 enables fast RESOLFT nanoscopy of living cells

    Tim Grotjohann et al.
    The discovery of a fluorescent protein that can be rapidly switched between long-lived ‘on’ and ‘off’ states will lead to a new generation of super-resolution imaging experiments on living cells.
    1. Cell Biology
    2. Developmental Biology

    Quantitative mapping of dense microtubule arrays in mammalian neurons

    Eugene A Katrukha et al.
    Quantitative super-resolution light microscopy reveals the relative abundance and three-dimensional organization of different microtubule subsets within dendrites of mammalian neurons.
    1. Cell Biology
    2. Developmental Biology

    Alstrom syndrome gene is a stem-cell-specific regulator of centriole duplication in the Drosophila testis

    Cuie Chen, Yukiko M Yamashita
    Alms1a is a centrosomal protein that exhibits asymmetric localization between mother and daughter centrosomes in asymmetrically dividing stem cells in Drosophila testis, controlling centriole duplication.
    1. Cell Biology
    2. Developmental Biology

    Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages

    Weng Man Chong et al.
    Super-resolved architectural framework of subdistal appendages surprisingly reveals coupling between distal appendages and subdistal appendages.
    1. Cell Biology

    Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes

    Natalia Wesolowska et al.
    Combined light and electron microscopy reveals a new function for Arp2/3-mediated actin assembly in nuclear envelope rupture, which leads to a separation of nuclear membranes and pores from the lamina.
    1. Physics of Living Systems

    Downsizing the molecular spring of the giant protein titin reveals that skeletal muscle titin determines passive stiffness and drives longitudinal hypertrophy

    Ambjorn Brynnel et al.
    Genetically altering the size of the molecular spring element in the giant protein titin established that titin determines the stiffness of skeletal muscle and the number of sarcomeres in series.

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