6,857 results found
    1. Neuroscience

    A robust activity marking system for exploring active neuronal ensembles

    Andreas T Sørensen et al.
    A new system for tagging activated neuronal population offers multiple advantages over existing systems based on immediate early genes, including greater sensitivity and specificity, and suitability for easy application in species other than mice.
    1. Stem Cells and Regenerative Medicine

    Loss of adult skeletal muscle stem cells drives age-related neuromuscular junction degeneration

    Wenxuan Liu et al.
    Building on previous work (Liu et al., 2015), it is shown that depletion or rescue of adult skeletal muscle stem cells is sufficient to induce or attenuate age-associated neuromuscular junction deterioration respectively.
    1. Ecology

    Acknowledging selection at sub-organismal levels resolves controversy on pro-cooperation mechanisms

    Wenying Shou
    Building on previous work (Momeni et al., 2013), it is shown that recognizing the hierarchical organization of biological systems resolves the ongoing controversy on pro-cooperation mechanisms.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

    Steven Lin et al.
    Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Assembling the Tat protein translocase

    Felicity Alcock et al.
    Evolutionary bioinformatics and experimentation are applied to the components of the Tat protein transport system to elucidate the structure of the membrane-bound receptor complex and to deduce a molecular description for its substrate-triggered activation.
    1. Neuroscience

    Need-based prioritization of behavior

    C Joseph Burnett et al.
    A rich behavioral data set reveals the complex interactions and influence motivational systems have on one another, driving competition and optimal expression of behavior.
    1. Biochemistry and Chemical Biology

    In vivo experiments do not support the charge zipper model for Tat translocase assembly

    Felicity Alcock et al.
    A prominent model for the structural organisation of the Tat (twin-arginine translocase) protein transport system fails live cell tests.
    1. Developmental Biology

    SOXF factors regulate murine satellite cell self-renewal and function through inhibition of β-catenin activity

    Sonia Alonso-Martin et al.
    A set of ex vivo and in vivo experiments, including genetic ablation and regeneration studies, identify a key regulatory function of SOXF factors in muscle stem cells in mice.
    1. Stem Cells and Regenerative Medicine
    2. Chromosomes and Gene Expression

    Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

    Yujia Cai et al.
    Building on previous work (Cai et al., 2014), HIV-derived lentiviral vectors are harnessed to insert user-defined genes into a safe locus in the genome of human stem cells.
    1. Stem Cells and Regenerative Medicine

    A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids

    Briana R Dye et al.
    Building on previous work (Dye et al., 2015) showing that pluripotent stem cell derived lung organoids were immature/fetal, the current study shows that in vivo transplantation leads to mature tissue, reminiscent of adult airways.

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