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    1. Biochemistry and Chemical Biology
    2. Medicine

    Framework for rapid comparison of extracellular vesicle isolation methods

    Dmitry Ter-Ovanesyan, Maia Norman ... David R Walt
    Measuring surface proteins on extracellular vesicles enables quantitative comparison of different isolation methods and optimization of size exclusion chromatography.
    1. Ecology
    2. Neuroscience

    Patterns of call communication between group-housed zebra finches change during the breeding cycle

    Lisa F Gill, Wolfgang Goymann ... Manfred Gahr
    Call-based vocal communication of individually recorded zebra finches changes in social groups across reproductive stages and is related with successful egg laying.
    1. Microbiology and Infectious Disease

    Evolution of a functionally intact but antigenically distinct DENV fusion loop

    Rita M Meganck, Deanna Zhu ... Longping V Tse
    Operating as a serum profiling tool for dengue infection and vaccination, the D2-FLM variant, which is insensitive to antibody-dependent enhancement-prone fusion loop antibodies, also offers potential for a safer dengue virus vaccine.
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    1. Neuroscience

    TIR-1/SARM1 inhibits axon regeneration and promotes axon degeneration

    Victoria L Czech, Lauren C O'Connor ... Alexandra B Byrne
    TIR-1 regulates both repair and destruction on either side of an injury axon.
    1. Neuroscience

    Somatic and vicarious pain are represented by dissociable multivariate brain patterns

    Anjali Krishnan, Choong-Wan Woo ... Tor D Wager
    Understanding others' pain is grounded in cognitive rather than sensory faculties.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization

    David Tse Shen Lin, Elizabeth Conibear
    Palmitate turnover is controlled by distinct enzyme families with different specificities.
    1. Immunology and Inflammation

    Regulation of positive and negative selection and TCR signaling during thymic T cell development by capicua

    Soeun Kim, Guk-Yeol Park ... Yoontae Lee
    Deficiency of capicua, a transcription factor that suppresses autoimmunity, impairs positive and negative selection processes by attenuating TCR signaling in CD4+CD8+ double-positive thymocytes.
    1. Neuroscience

    The shared and unique neural correlates of personal semantic, general semantic, and episodic memory

    Annick FN Tanguay, Daniela J Palombo ... Louis Renoult
    Four types of declarative memory rely on different weightings of similar component processes, according to fMRI and subjective ratings.
    1. Plant Biology

    Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling in Arabidopsis

    Pengfei Cao, Sang-Jin Kim ... Federica Brandizzi
    Despite BCAAs being de novo-synthesized in chloroplasts, TOR activation by BCAAs is conserved in plants and triggers a re-organization of actin and actin-associated endomembranes.
    1. Structural Biology and Molecular Biophysics

    Direct single molecule measurement of TCR triggering by agonist pMHC in living primary T cells

    Geoff P O'Donoghue, Rafal M Pielak ... Jay T Groves
    Quantitative observations of single-molecule binding between antigen and T cell receptor (TCR) in living primary T cells reveals unbinding kinetics, stoichiometry and signaling molecule recruitment, providing insights into the mechanisms of antigen recognition by the immune system.